Flow Cytometry:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Recombinant:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Virus:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Magnetic Beads:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Protease Inhibitor:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
CCK-8 Assay:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Modification:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Staining:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
Milk:Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER APC/Cyanine7 anti-mouse CD45 (Flow cytometry) BioLegend Cat# 103116; RRID: AB_312981 PE anti-mouse IFN-γ (Flow cytometry) BioLegend Cat# 505808; RRID: AB_315401 APC anti-mouse CD45 Recombinant (Flow cytometry) BioLegend Cat# 157605; RRID: AB_2876537 PerCP/Cyanine5.5 anti-mouse CD3Ɛ (Flow cytometry) BioLegend Cat# 100328; RRID: AB_893318 Brilliant Violet 421TM anti-mouse CD4 (Flow cytometry) BioLegend Cat# 116023; RRID: AB_2800579 FITC anti-mouse CD8α (Flow cytometry) BioLegend Cat# 100706; RRID: AB_312745 PE anti-human/mouse Granzyme B Recombinant (Flow cytometry) BioLegend Cat# 372208; RRID: AB_2687032 APC anti-mouse Perforin (Flow cytometry) BioLegend Cat# 154404; RRID: AB_2721465 FITC Rat Anti-Mouse CD4 (Flow cytometry) BD Cat# 553729; RRID: AB_395013 Bacterial and Virus Strains E coli Trans109 Lab preserve N/A Chemicals, Peptides, and Recombinant Proteins Paroxetine Hydrochloride MCE Cat# HY-B0492 Antidepressant Compound Custom Library TargetMol Rack ID:PHD163731/PHD163732/PHD163733 Puromycin Gibco Cat# a1113803 Z-DEVD-FMK MCE Cat# HY-12466 Serotonin MCE Cat# HY-B1473A Necrosulfonamide MCE Cat# HY-100573 Ferrostatin-1 MCE Cat# HY-100579 protein A/G magnetic beads MCE Cat# HY-K0202 16% formaldehyde methanol-free Thermo Cat# 28908 Protamine sulfate AMRESCO Cat# 0382 Protease Inhibitor Cocktail (PI) APExBIO Cat# K1007 Disuccinimidyl glutarate (DSG) Proteochem Cat# C1104 Crystal violet Sigma Cat# C6158-100G Cell Counting Kit-8 Targetmol Cat# C0005 Ampicillin sodium Sangon Cat# A610028-0025 N,N,N ′ ,N-Tetramethylethylenediamine (TEMED) Sigma Cat# T7024 Penicillin G sodium salt Sangon Cat# A600135-0100 Streptomycin sulfate Sangon Cat# A610494-0250 FastDigest BshTI Thermo Scientific Cat# FD1464 FastDigest EcoRI Thermo Scientific Cat# FD0275 30% Acr-Bis (29:1) Biosharp Cat# BL513B Ammonium persulfate Sigma Cat# A3678 Luminol Sigma Cat# A8511 4-lodophenylboronic Sigma Cat# 471933 Dimethyl sulfoxide MCE Cat# HY-Y0320 Dulbecco’s Modified Eagle Medium Gibco Cat# 12800-017 Fetal Bovine Serum Gemini Cat# 900-108 PageRuler Prestained Protein Ladder Thermo Scientific Cat# 26617 GoldBand 1 kb DNA ladder Yeasen Cat# 10510-A YeaRed Nucleic Acid Gel Stain Yeasen Cat# 10202ES76 NON-Fat Powdered Milk Sangon Cat# A600669-0250 Agarose M Sangon Cat# A610013-0250 Agar BioFroxx Cat# 8211GR500 Albumin, Bovine (BSA) Amresco Cat# 0332 (Continued on next page) e2 Cell Reports Medicine 7, 102537, January 20, 2026 OPEN ACCESS
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